Anne is seen face first in a puddle, then she stands up to realize she is not home. The cam pans out to show a lily-pad shaped island.
Creatures, Amphibians, and more are seen as the cam passed to Anne ducking under the Plantar family house's small door and standing straight up with her hands on her hips. She is interrupted by a bunch of dragonflies zooming over her head.
The cam follows the dragonflies to an upper window which opens and introduces Sprig Plantar, who trips out the window. The cam enters a room where Hop Pop Plantar is reading and looks out the window to see Sprig falling for his life. Sprig lands in a wooden bucket then hit in the air by his younger sister who uses the bucket to stay cool in her water.
Sprig joins Anne and points the way. Anne and Sprig travel to various locations in Amphibia and meeting different characters. Hop Pop and Polly appear in some scenes.
An old printed image appears of the same three human girls from the first shots with the image being subtitled with "BFFs!!" and a heart. It appears to be stuck in the branch of a tree while the wind blows it as it storms.
The Amphibia logo appears then a shine streaks across it with nature background sounds. The theme ends there. The short version also revolves around this, though the nature sounds are absent and the camera pans differently. Behind the logo is a stone carving of Anne, Sasha, and Marcy all armed with weapons fighting against a giant toad-like creature (Possibly Grime) with flame in his hands.
The instrumental theme does not follow the same melody as that of Welcome to Amphibia, rather a different rendition of the same theme.
The Plantar Farm door and window effects, as well as the yellow terrain and the boulder effects, are animated in CGI.
Footage of the chest opening and the three girls being transported to Amphibia is used in Best Fronds.