Humans (Homo sapiens) are the only surviving members of the subtribe Hominina. Together with chimpanzees, gorillas, and orangutans, they are part of the family Hominidae. A terrestrial animal, humans are characterized by their erect posture and heavy tool use, as well as complex language compared to other animals. They live in highly advanced and technologically developed societies.
Being new to Wartwood, the amphibians feared Anne due to her being a species they had never encountered before. Grime was also highly suspicious of Sasha initially due to similar reasons and questioned her background. However, the rest of the Valley has not yet been explored in the series and it is likely that there are amphibians who have encountered or have knowledge of humans. One such being Valeriana who owned a stall in the Bizarre Bazaar selling artifacts and relics she had acquired from her years of travel throughout Amphibia. When Anne asked Valeriana if she had seen anything like her, she replied "Not exactly" which indicates that she might have known a human but of different features, like race, skin color, height, hair color, etc.